|
|
|
 |
|
|
|
Cheap and Easy Operon
|
|
| |
by Patty Young
Lutheran High School of Orange County
Orange, California
|
|
| | Materials
- cardboard tube from middle of paper towel or wrapping paper roll
- colors of construction paper -- cut into 2-inch strips
- black felt pen
- Tape
- foam insulation for pipes -- cut into 2-inch sections, two pieces for eachstudent
- pop-it beads from mitosis kit
Procedure
- Each student cuts one piece of construction paper and wraps it around the cardboard tube. This strip should be "upstream" from the operon, labeled RG for regulatory gene.
- Tape five other pieces of construction paper close to each other on the tube, labeling them P for promoter, O for operator, and three Gs for gene -- these should each be a different color except for the genes, which should all be the same color.
- One 2-inch section of foam should be labeled RNA polymerase, and the other should be labeled Repressor, assuming this is a tryp repressor model. It can be easily modified to be any type of operon as well.
- Use the pop-it beads to show the products of the genes as RNA polymerase transcribes them.
|
|
|
|
|
|
Course Home Pages